Modern NMR spectroscopy has been emphasizing the application in biomolecular systems and plays an important role in structural biology. Cade-Menun provided an essential overview of the most important studies on the application of solution-state 31 P NMR spectroscopy for the characterization and quantification of P forms in non-pasture cropland soils. The integration of results from these different techniques enabled the authors to propose a model structural unit for the specific suburban from Betula nigra bark under study. A study on complexes between calcium-exchanged montmorillonite and either fulvic acids or soil alkaline extracts was conducted by 1H HRMAS NMR and showed that aliphatic and, partially, aromatic components bound preferentially to the clay surface [20]. Already in 2004, HRMAS was applied to trace the metabolomic profile of the Italian Parmigiano Reggiano cheese [64] and to correlate its concentration of several free amino acids and low molecular-weight metabolites to the cheese ripening during a 24 months-long monitoring [65]. Remarkably, they exploited the 13C labeling of plants by making use of 3D experiments, such as 1H-13C HCCH-COSY and 1H-13C HCCH-TOCSY, to unambiguously assign several signals. 2014;13:3075–87. Starch granule hydration—a MAS NMR Investigation. Deshmukh AP, Simpson AJ, Hadad CM, Hatcher PG. J Braz Chem Soc. Lam et al. This work also showed that the biomass degradation pathways are similar to those of plant material under similar degrading conditions, thus suggesting that the difference between recalcitrant carbon from different sources is negligible after decomposition. Biol Fertil Soils. Ritota M, Comarini F, Sequi P, Valentini M. Metabolomic characterization of Italian sweet pepper (Capsicum annum L.) by means of HRMAS-NMR spectroscopy and multivariate analysis. Here, we described the most relevant applications of HRMAS in studies related to agricultural issues. Another study combined HRMAS and DRIFT (Diffuse reflectance infrared Fourier transform spectroscopy) techniques to identify the relationship between the structure of soil humic substances and long-term (40 years) soil management practices, including organic and/or mineral fertilizations [25]. High resolution Proton, Carbon-13 and nitrogen-15 nuclear magnetic resonance studies of when proteins at high magnetic fields: Spectral assignments, changes with concentration and heating treatments of flinor gliadins in solution-comparison with gluten spectra. MRI is an application of NMR (nuclear magnetic resonance), an analytical tool of chemists found in laboratories worldwide. Physical, chemical, and biochemical mechanisms of soil organic matter stabilization under conservation tillage systems: a central role for microbes and microbial by-products in C sequestration. 2013;61:4979–87. Biol Trace Elem Res. Larsen FH, Blennow A, Engelsen SB. Šmejkalová D, Spaccini R, Piccolo A. Multivariate analysis of CPMAS 13C-NMR spectra of soils and humic matter as a tool to evaluate organic carbon quality in natural systems. Comprehensive multiphase NMR spectroscopy: basic experimental approaches to differentiate phases in heterogeneous samples. In addition, in the process of the grains and oil’s production, the oil content of oilseeds, oilseed residue and feedstuff is very important. It can find applications in agriculture for the quality analysis of fruits and vegetables at the industrial or semi-industrial level. The evident versatile potential of the HRMAS NMR makes this technique particularly useful for life science molecular studies. In vivo 31P and 1H HR-MAS NMR spectroscopy analysis of the unstarved Aporrectodea caliginosa (Lumbricidae). A 1H HR-MAS NMR-based metabolomic study for metabolic characterization of rice grain from various Oryza sativa L. cultivars. The HRMAS becomes ever more important for the investigation of metabolic processes in plants as related to specific management practices or to responses to either abiotic stress or phytopathogens infections. The HRMAS technique has been also applied to evaluate transgenic plants. 2015;53:735–44. Use of arsenic contaminated irrigation water for lettuce cropping: effects on soil, groundwater, and vegetal. PubMed  While the 1D 13C spectra of fresh plant tissues well differentiated the composition of fresh pine and grass materials, the differences became negligible after decomposition and suggested a similar accumulation of recalcitrant carbon species, thus again supporting a mechanism of progressive selection of hydrophobic degradation products regardless of the plant origin [21]. 2010;232:85–93. Parmigiano Reggiano as a case study. Interestingly, this work showed that HRMAS applied to the whole NOM samples was superior to the traditional liquid-state NMR measurements of extracts from the same NOM materials [24]. They focused on the biocontrol of yeast Pichia anomala J121 and verified how their intracellular metabolites varied as a function of an induced oxygen limitation. In soil science dose-dependent response only in pear plants [ 45 ] same time, the abiotic stress the... Interesting works have described the application in biomolecular systems and plays an important role in biology! 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